Buchholz started Wednesday's 6-4 loss to the Oakland Athletics but struggled, giving up two home runs and failing to record an out in the fifth inning. He took the loss and was charged with five earned runs on six hits and three walks versus just two strikeouts.
Lowrie played shortstop and batted second, going 1-for-2 with an RBI single, two walks and one run. He committed a throwing error in the fourth inning, allowing Rajai Davis to reach first.
Lowrie rejoined the club Tuesday after being out all season with mononucleosis. He now has no limitations and he said he feels healthy and ready to contribute.
"I'm just looking to play baseball and not have to think about all this," Lowrie said Tuesday. "I want to put it all behind me and just let my ability play."
To make room for Lowrie the Red Sox designated first baseman Ryan Shealy for assignment. Shealy went 0-for-7 for Boston.
Additionally, the team optioned reliever Dustin Richardson to Triple-A Pawtucket to make room for Buchholz. Richardson allowed two runs in seven innings over 13 relief appearances in the majors.
The 26-year-old Lowrie was limited to 32 games last season after having surgery on his left wrist to repair a torn ligament in April 2009. He was placed on the disabled list while the team was in Oakland at the start of last season.
He first suffered the wrist injury in May 2008 and played through the discomfort. He was deemed healthy in time for spring training in 2009, but the wrist began to aggravate him again toward the end of camp before it was finally decided he would have the surgery.
Lowrie returned to the Red Sox's lineup on July 18, 2009, at Toronto and played in 14 games before returning to the DL. He was reinstated on Sept. 8 and finished the season on the active roster.
During the offseason, the Red Sox signed shortstop Marco Scutaro and Lowrie felt he would battle for the starting job during spring training before mono completely shut him down.
"I've been asked about timetables and percentages for the last two years," Lowrie said. "I feel healthy and that's good enough for me right now.
"If there's a silver lining in all this, the wrist feels pretty good," he said.
Information from ESPNBoston.com's Joe McDonald and The Associated Press was used in this report.