Alfonso Soriano, who was on the disabled list with a strained right quad, will take Barney's place on the roster. Soriano is not in the starting lineup Wednesday, but he's available for pinch-hitting duties.
Barney injured the knee scoring the only run of Monday night's game, sliding home head first to avoid a tag. He said that the knee could be better in five-to-seven days, but it's best for he and the team that he goes on the DL.
"As soon as I slid into home plate last night, I knew something was really wrong," he said. "The whole knee went numb. I had an MRI today and it revealed that Grade 1 strain in my knee."
Barney's is the latest injury in a season full of them for the Cubs. In addition to Barney and Soriano, Andrew Cashner, Randy Wells, Marlon Byrd, Jeff Baker, Reed Johnson, Kerry Wood and Geovany Soto have spent time on the DL this season.
Barney, a candidate for NL Rookie of the Year, was hitting .294 with one home run and 25 RBIs for the Cubs.
Bruce Levine cover the Cubs for ESPNChicago.com. ESPNChicago.com's Sahadev Sharma contributed to this story.