Coach Lincoln Riley said "early indications are positive" that the injury is not serious.
"We'll look at Trey Sermon. I don't think it's serious. We'll evaluate him further," Riley said.
Sermon was injured on a tackle by safety Patrick Fields and was helped off the field after an 11-yard run.
Sermon rushed for 744 yards and five touchdowns and caught 16 passes for 139 yards and two more scores as a freshman this past season. He's part of perhaps the team's deepest position. Rodney Anderson is the team's top returning rusher.
The Sooners dress in full pads and tackle during their spring game. The quarterbacks wear blue jerseys and are down by touch, but the rest of the players can be tackled.
Information from the Associated Press was used in this report.