Athletics spokesman Kirk Sampson said Wednesday that Blakely is no longer enrolled. Blakely initially signed with Florida but transferred a few months later and sat out the 2011 season.
He played in six games for the Tigers and was third on the team with 153 yards on 33 carries.
Junior-college transfer Cameron Artis-Payne has signed to play for Auburn, which is expected to sign at least one more back on national signing day next Wednesday.
The Tigers averaged just 148 rushing yards after Mike Dyer left the team and signee Jovon Robinson was declared ineligible.