Nicknamed "Mr. Marlin," Conine will sign a one-day contract on March 28 when the Marlins face the New York Yankees in an exhibition game at Dolphin Stadium, then retire.
Conine, 41, was an original member of the team's inaugural roster and had two stints with the organization. As a first baseman and outfielder, Conine played a key role in the team's 1997 and 2003 World Series titles.
The Marlins also plan to honor Conine in an on-field ceremony in their season opener against the New York Mets on March 31.
Conine compiled a .285 career batting average with 214 home runs and 1,071 RBIs. He is the Marlins leader in career grand slams with six, and he's second in team history in total hits (1,005), singles (688), RBIs (553), total bases (1,579) and games played (1,014).