Nolasco (14-9, 4.51) was evaluated after experiencing some discomfort. He said he will undergo surgery next week.
"It's just not letting me do what I want to do," he said Wednesday.
Hayes was injured on an inning-ending double play in the 10th inning Tuesday night when Washington's Nyjer Morgan slammed into him trying to score from second base on a ground ball.
The Marlins called up catcher Chris Hatcher from Double-A Jacksonville. Florida also called up right-hander Jorge Sosa from Triple-A New Orleans.