Maybin ruptured his left biceps tendon on March 2 when he robbed the Dodgers' Juan Uribe of extra bases with a diving catch in a spring training game. Maybin didn't undergo surgery.
He batted seventh Sunday and finished with two hits, including a double to left in his first at-bat. To make room for Maybin on the roster, the Padres sent Kevin Quackenbush to Triple-A El Paso.
Maybin went 9-for-37 with a home run in 10 rehab games, according to U-T San Diego. Maybin said before Sunday's game that his "whole body's feeling good" after the rehab assignment, according to the newspaper.
"This is where I belong, and it's good to be back, get ready to play some ball," he said, according to the newspaper.
A year ago, the Padres center fielder tore the left posterior cruciate ligament, a rare knee injury. When he returned, he hurt his wrist and needed surgery again, ending his season after 13 games.
The Padres thought they had their center fielder of the future in 2012 when they signed Maybin, then 25, to a five-year, $25 million contract. He had hit a career-best .264 with 40 stolen bases in 2011 and was starting to show the potential that led Detroit to take the Asheville, N.C., native with the 10th pick in the 2005 draft.
Maybin played 147 games in 2012, hitting .243 with 26 steals before all the injuries hit.
The Associated Press contributed to this report.