Tulowitzki was running out a ground ball to shortstop in the top of the fourth inning when he grabbed his left leg halfway down the first base line. He was thrown out easily and gingerly made his way to the dugout before being replaced by Josh Rutledge.
The Rockies lost the game, 3-2 in 11 innings.
"Nothing serious," Tulowitzki said. "We'll see how I wake up (Sunday). The good news is I don't think I tore anything or did anything to where I'm going to miss an extended period of time."
Tulowitzki was not in the lineup for Sunday's 5-3 loss against Pittsburgh, which capped a three-game sweep.
The 29-year-old Tulowitzki, who has battled injuries in the past, is in the middle of a career season.
He began play Saturday leading the major leagues with a .342 batting average. His 71 runs scored are also tops in the majors.
The Associated Press contributed to this report.