Bautista, who leads the majors with 31 homers this season, hurt himself in Toronto's eventual 16-7 victory over the New York Yankees. He limped off under his own power after the play in the fourth inning.
Bautista was getting treatment after the game and did not speak to the media, but Blue Jays manager John Farrell said his All-Star slugger caught a spike in the dirt.
"Hopefully this isn't a prolonged situation where he's going to miss any length of time," Farrell said.
Farrell said X-rays were negative, adding that Bautista might undergo an MRI Friday.
Bautista, who led the big leagues last year with 54 homers, was hurt going into third base on Edwin Encarnacion's double-play grounder that ended the inning. Bautista stayed on his knees and was tended to by trainer George Poulis and Farrell.
"You never want to see anybody get hurt, especially our leader in Jose," catcher J.P. Arencibia said. "I didn't get to see what happened, I just saw him in pain on the (turf). He's a fighter and he'll be back as soon as possible."
Information from The Associated Press was used in this report.