Peralta was ejected from Tuesday night's game in Washington after umpires found pine tar on his mitt.
The incident led to a verbal spat between managers Joe Maddon of the Rays and Davey Johnson of the Nationals.
Peralta said he planned to appeal Major League Baseball's decision after the suspension was announced Thursday.
Peralta then served up a tiebreaking, two-run double to Danny Espinosa in the sixth inning Thursday night, sending the Nationals over the Rays, 5-2.
Andrew Friedman, Tampa Bay's executive vice president of baseball operations, talked to reporters about Peralta's decision before Friday's scheduled Rays-Phillies game at Citizens Bank Park.
"It had kind of been weighing on Joel," Friedman said. "The thought was to go ahead and knock this out and get it behind him, and it will be better for everyone."
Information from ESPN.com's Jerry Crasnick was used in this report.