The reigning AL MVP and Triple Crown winner had missed three straight games with what the team called a strained abdomen, but he returned for Tuesday's 2-1 loss to Boston and went 0 for 4.
Manager Jim Leyland told reporters "he's a little sore" before the lineup was posted in the locker room.
Cabrera leads the majors with a .355 average with 130 RBIs. He has 43 homers, four behind the Orioles' Chris Davis.
Don Kelly is scheduled to play third and bat sixth.