Chalmers, who has missed the last three games with a sprained ankle, did participate in the team's Friday morning shootaround.
The Heat can afford to rest most of their stars after locking up the No. 1 seed in the Eastern Conference.
This will be the third straight game James and Wade have missed. James is battling tightness in his right hamstring, while Wade is nursing several minor injuries in his leg.
Allen is out after tweaking his right ankle when he fell over a cameraman during the fourth quarter of Tuesday night's game against the Knicks.