There had been question as to whether Pettitte would start for Double-A Trenton or Tampa. Trenton is playing in Maine, so Pettitte will stay out of the cold and pitch in Dunedin instead.
Pettitte has made five tuneup starts so far: One for Trenton, two for Tampa and also in an extended spring training camp. He threw 81 pitches over five innings last time out and is expected to throw 90 to 95 pitches on Monday.
Pettitte is expected to make at least two more starts before a return to the majors.
"He feels good," Yankees manager Joe Girardi said of Pettitte. "That's the big thing, that he physically feels good and he doesn't feel like it's taxing his body too much. ... He's bouncing back really well."
Mike Mazzeo is a regular contributor to ESPNNewYork.com. Matt Ehalt of ESPNNewYork contributed to this story.