Both players were listed as active and were in the lineup when the Giants won 20-17, keeping the Dolphins winless.
Tuck, who hadn't played since Sept. 25 in Philadelphia, said he expected to be back following the team's Week 7 bye. He's missed the last three games with neck and groin injuries. Tuck had been listed as questionable. He practiced on a limited basis all week.
"Obviously there's fear," Tuck said of possibly re-injuring himself. "Anytime you have any little tweak in anything, you're kind of self-conscious about it. But it's the game we play."
Jacobs had missed the last two games due to a sprained medial collateral ligament. He was listed as probable and said Thursday he felt "explosive" in practice.
"I'm ready to get out there and get some snaps and play," Jacobs, who hasn't played since Oct. 2, said Friday.
The Giants now find themselves quite deep at defensive end, with Tuck joining Jason Pierre-Paul, Osi Umenyiora and Dave Tollefson. It is likely that Tuck will start opposite Umenyiora, meaning Pierre-Paul will move into a reserve role, though the second-year pro will see plenty of snaps given that his 7½ sacks rank third in the NFL behind Minnesota's Jared Allen and Dallas' DeMarcus Ware.
Jacobs will see carries behind starter Ahmad Bradshaw. The 29-year-old running back made headlines earlier in the week when he told Men's Fitness Magazine that he hasn't been happy with his role. Jacobs has averaged just 9.5 carries per game so far, but he's only gained 3.1 yards per carry.
Inactive for the Giants Sunday were rookie cornerback Prince Amukamara, fullback Henry Hynoski, offensive linemen Shawn Andrews, James Brewer, Mitch Petrus, defensive tackle Dwayne Hendricks and running back Da'Rel Scott.
Mike Mazzeo is a regular contributor to ESPNNewYork.com. Information from The Associated Press was used in this report.