All three players were ruled out on Wednesday, along with rookie defensive end Adewale Ojomo, and will not make the trip to Charlotte.
Wide receiver Hakeem Nicks and cornerback Michael Coe have been listed as questionable for Thursday's game. Linebacker Keith Rivers and cornerback Prince Amukamara have been listed as probable -- meaning Amukamara likely will make his season debut after missing the first two weeks with a high ankle sprain.
Bradshaw, the team's starting running back, injured his neck in the second quarter of the Giants' 41-34 win over the Tampa Bay Buccaneers on Sunday and did not return. The Giants have provided no further details on his injury. Andre Brown, who performed well in Bradshaw's place -- 13 carries for 71 yards and a touchdown -- likely will get his first NFL start.
Diehl, the team's starting right tackle, injured the MCL in his right knee against the Bucs. His status beyond this week is also unknown. The Giants shifted starting left tackle Sean Locklear to right tackle and inserted Will Beatty at left tackle after Diehl went down. The same configuration is likely on Thursday night.
Hixon, the team's third wide receiver, suffered a concussion Sunday, also in the first quarter. Fourth-year wideout Ramses Barden is the most likely candidate to take Hixon's spot, although rookie Rueben Randle could get some snaps in that position as well.