Starting running back Marshawn Lynch and two-time Pro Bowl left tackle Jason Peters both missed practice because of injuries, but coach Dick Jauron is optimistic they'll both play in the finale against New England.
Lynch is nursing a sore shoulder that kept him out of most of the second half of Buffalo's win at Denver last Sunday, while Peters has a knee injury that forced him to miss the Broncos game.
"You never know how the week's going to work out, and there's no way to really tell. But both guys are tough guys and they'll go if they can," Jauron said.
Lynch has accumulated 1,036 yards rushing and eight touchdowns on the ground this season. His backup, Fred Jackson, has added 435 yards rushing, including 43 at Denver when he came in for Lynch.
The pair has also combined for 83 catches.
"We'll see what happens on how Marshawn's shoulder is going to hold up," Jackson said. "You have to be ready if you're called upon. That's how I'm approaching it."
Starting linebacker Kawika Mitchell (knee), receiver Roscoe Parrish (knee), and linebacker Teddy Lehman (groin), also didn't participate in practice, while starting cornerback Jabari Greer (knee) was limited. Greer has missed the last five games.
Sunday's game has significant playoff implications for the Patriots (10-5), who must win to stay in the postseason hunt. The Bills (7-8) will be looking to finish .500 for the first time since going 9-7 in 2004.