Coach Jim Schwartz said Stafford won't be healthy enough to play Sunday and Hill will make his second-straight start.
Stafford was knocked out of the season opener by Chicago's Julius Peppers, putting him on the sideline with a banged-up right shoulder -- his third injury in two seasons. Stafford missed six games last year after being the No. 1 pick in the draft because of a banged-up left shoulder and right knee.
Detroit is 0-3 this season and 3-40 since midway through 2007, giving the Lions the worst 43-game mark in NFL history, according to STATS LLC.
Stafford told WJR-AM on Monday that he would likely test his injured right shoulder with some throwing this week. He didn't throw any passes Wednesday during the part of practice open to the media.