The DA's office announced its decision Monday, saying in a statement that it "lacked sufficient evidence" to charge Agholor.
"The District Attorney's Family Violence and Sexual Assault Unit and detectives from the Philadelphia Police Department's Special Victims Unit thoroughly investigated the allegations of sexual assault against Nelson Agholor," the statement said.
"As a result of this joint investigation, our staff and the Police reached the conclusion that we lacked sufficient evidence to bring charges against Mr. Agholor, therefore no charges will be filed."
A dancer at Cheerleaders, a South Philadelphia strip club, accused Agholor of sexual assault in June. Agholor and teammates Fletcher Cox and Connor Barwin were in the club shortly after the final practice of the Eagles' offseason minicamp.
Cox and Barwin released statements saying they were at Cheerleaders but left before the alleged incident occurred.
Police turned the investigation over to the DA's office three weeks ago. Agholor's Philadelphia-based attorney, Fortunato Perri Jr., reiterated to ESPN's John Barr that the second-year receiver "didn't commit a crime."
"I applaud the detectives in the Special Victims unit as well as the District Attorney's office for conducting a full and exhaustive investigation into the allegations," Perri told Barr. "We said from the outset that Nelson didn't commit a crime and the result of the investigation now confirms the same conclusion."
Agholor, 23, was the Eagles' first-round draft choice in 2015.