Matthews suffered the injury Sunday, less than two days after the Bills acquired the wide receiver in a trade with the Philadelphia Eagles.
Bills general manager Brandon Beane told WGR 550 on Monday that Matthews suffered the injury early in practice when he went up for a contested ball and came down hard. Matthews finished the session and later left the field with a trainer. He was not made available to reporters because of the injury.
Matthews took first- and second-team reps Sunday and saw his first work with quarterback Tyrod Taylor since the Bills traded cornerback Ronald Darby to the Eagles for Matthews and a 2018 third-round pick. In a separate deal Friday, Buffalo dealt top receiver Sammy Watkins and a 2018 sixth-round pick to the Los Angeles Rams for a 2018 second-round pick and cornerback E.J. Gaines.
Coach Sean McDermott said before Sunday's practice that he would like to have Matthews play in Thursday's preseason game in Philadelphia.