The Panthers have placed starting cornerback Captain Munnerlyn on injured reserve with a strained left hamstring.
Munnerlyn was injured last week against the Texans and had to leave the game.
Darius Butler, acquired off waivers earlier this season from the Patriots, will start Saturday against the Bucs, coach Ron Rivera said.
R.J. Stanford will be the team's nickel back.
Munnerlyn had started all 14 games for the Panthers.
The Panthers signed cornerback Jonathan Nelson from the practice squad to take his place.
The Panthers are also likely to be without starting strong safety Charles Godfrey, who is out with a strained right shoulder. He's listed as doubtful after missing practice again on Friday. If Godfrey can't start, then Jordan Pugh will get the nod.