The city will distribute Smith's donation to help victims cover their medical expenses.
"As a father and husband I cannot imagine the pain and suffering the victims are going through," a statement from Smith read on ProFootballtalk.com. "My family's hearts and prayers are extended to theirs, and I hope this contribution might assist in paying some of the medical bills that will help allow these families to move forward in this tragic circumstance."
The shooting, which occurred at a midnight premiere of the Batman film "The Dark Knight Rises," killed 12 and left scores of others wounded.
"Hopefully, this helps a little bit," Smith said. "From one NFL city to another, God bless."