In its postgame report, KCTV aired video of Charles waiting for Manning as he departed the stadium to get his autograph. Manning rallied the AFC West-leading Denver Broncos to their eighth win of the season as the last-place Chiefs dropped their eighth straight game.
"My mom wanted this autograph," he told the television station.
Charles, in a tweet Tuesday afternoon, thanked his Twitter followers for their support by writing, "Thank for the support and relax on the P Manning story, just two warriors showing each other respect..."
Receiver Dwayne Bowe, meanwhile, got his picture taken with Manning after Sunday's game.
"It's Peyton Manning," he said. "Who wouldn't want a picture with him?"
Bowe, who is scheduled to be a free agent after the season and is playing this year under the Chiefs' franchise tag, was asked whether he'd like to catch passes from the future Hall of Famer as well.
"That, too, but right now the best thing I have is a picture," he told KCTV.
Charles had 23 rushes for 107 yards in the Chiefs' 17-9 loss. Bowe caught four passes for 41 yards.
Former Chiefs player Rich Baldinger, who was in KCTV's studio after the segment aired, took offense to Kansas City's two biggest offensive stars' actions.
Baldinger, a former offensive lineman who played in 12 NFL seasons, said he never asked for an autograph during his playing career.
"I don't understand it at that moment," he said. "They were smiling and laughing after the loss like that today. I just think it just goes to show what this team's about. I don't know if winning's really that important."
Charles, however, got support from another top NFL running back Tuesday.
Texans running back Arian Foster tweeted that he got Charles' autograph at the 2010 Pro Bowl, and that he exchanges with players "damn near every game."
Information from The Associated Press was used in this report.