There was a moment of silence before the national anthem while the names and ages of each victim were shown on the videoboards at Ford Field.
The decals on the back of the helmets read "S.H.E.S." in white on a black background.
The Lions' coaches wore pins with a similar design.
Twenty children and six adults were killed in the Dec. 14 shooting in Newtown, Conn. Adam Lanza killed his mother, attacked the school, then killed himself.
Also Saturday, the Bridgeport Sound Tigers, the New York Islanders' minor league affiliate, wore jerseys that replaced the players' last names with each of the 20 Newtown children.
The arena is located about 20 miles from Newtown.
Information from The Associated Press and ESPN.com's Darren Rovell was used in this report.