Tuesday's not guilty plea came a day after the 29-year-old Jones was arrested and then released on his own recognizance.
Jones is accused of hitting a 34-year-old woman at a downtown Cincinnati nightclub Wednesday shortly before midnight. A police report lists the injury as apparently minor.
Jones' agent, Peter Schaffer, told ESPN NFL Insider Adam Schefter on Monday that Jones was approached by two intoxicated women who wanted to have their picture taken with the football player last Wednesday. When Jones refused, one of the women threw a beer bottle at him and Jones responded by allegedly slapping her.
Surveillance video of altercation, obtained by a local radio station and verified as authentic by Cincinnati police, appears to show Jones approaching two seated women outside the club. Then, a third woman approaches Jones and appears to confront him. The grainy video shows the woman reach toward Jones' face but it's unclear what she does or whether she's holding anything in her hand.
Jones then appears to hit the woman, who falls to the ground but quickly gets up and follows Jones off-camera as another man with him ushers him away.
Jones wrote on his Twitter account Monday that he was protecting himself.
"Just got arrested for protecting myself, I will not let this break me or change what I have work so hard for," Jones tweeted.
On Monday, NFL spokesman Greg Aiello told ESPN, "we're aware of it and will review the facts at the appropriate time."
Jones practiced with the team Tuesday as it began a mandatory three-day minicamp. He declined to comment on his arrest.
"The situation Adam Jones is in is not something you want to get involved in," Bengals coach Marvin Lewis said. "It's unfortunate that he's in this situation. That's really all I can add to it. Obviously, this has to work its way out, whichever way it does."
Jones' most recent encounter is not first time he has been in trouble. He pleaded guilty in January 2012 to a misdemeanor charge of disorderly conduct. He had been accused of shouting profanities and trying to pull away as police arrested him at a Cincinnati bar months earlier.
Also last year, Jones was ordered to pay $11 million in damages to two Las Vegas strip club employees injured in 2007 when a gunman claiming he was doing Jones' bidding opened fire outside the club after Jones and his entourage were kicked out. A club manager was paralyzed from the waist down and a bouncer was wounded.
Jones said he had no role in the shooting and pleaded an equivalent of no contest to misdemeanor conspiracy to commit disorderly conduct. The gunman, Arvin Kenti Edwards, is serving four to 10 years in prison.
Information from ESPN NFL Insider Adam Schefter, ESPN.com AFC North blogger Jamison Hensley and The Associated Press was used in this report.