Fisher sustained the concussion late in the first half of last Sunday's game against the New York Giants. He didn't play in the second half or practice this week.
Fisher, the top overall pick in this year's draft, will be replaced at right tackle Sunday by Donald Stephenson, who started seven games at tackle as a rookie last season.
Both players missed practice time this week, Charles with blisters on his feet and Flowers with inflammation in his right knee. Flowers didn't play against the Giants because of soreness in the knee, but he said Friday he was far ahead physically compared to where he was a week ago.
"Last week I kind of knew I wasn't going to play," he said. "I'm definitely going to play this week. Not playing last week really helped me out."