Johnson, whose right groin was injured during a loss to the Pittsburgh Steelers last Sunday, has been hampered by several injuries this season. This is the second game he will miss.
Playing in nine games, Johnson leads the Bills (3-7) with 41 receptions for 471 yards and three touchdowns.
In addition to Johnson, the Bills officially declared rookie receiver Robert Woods (ankle) out for Sunday's game. Woods also did not practice this week.