Matthews has missed the team's last 10 games with a right shoulder injury.
"In my mind, I think I'm ready to go, and taking it as I'm getting ready to play [Thursday]," Matthews told reporters after practice Wednesday.
"It felt good, nice to get in all the reps and everything. [Wednesday] was a good step forward in that process, going through the line rushes."
Matthews has 28 goals and 22 assists in 53 games this season for Toronto.