Henry Trinder and Marland Yarde have been added to the squad for England's training camp in Brighton this week.
Eddie Jones named a 31-man squad for the week-long camp last Thursday, which is preparation for the Twickenham clash with the Barbarians on May 27 and does not include any players involved in this weekend's Aviva Premiership semifinals.
London Irish fullback Ben Loader has since been forced to withdraw with an ankle injury, however, while a knee issue is expected to prevent Sale youngster Cameron Redpath from taking part.
"Henry Trinder (Gloucester Rugby) and Marland Yarde (Sale Sharks) have been called up into England's training squad for this week's camp in Brighton," a statement issued by the Rugby Football Union read.
"Ben Loader (London Irish) picked up an ankle injury on Friday and will remain with the England U20 squad for further treatment.
"Cameron Redpath (Sale Sharks) will report to camp on Monday for treatment on a knee injury and is not expected to train this week."
Jones will finalise his squad for the Barbarians game following this weekend's Premiership playoffs. Twenty-one players selected to tour South Africa next month will be involved in the semifinals.