Juve boss Massimiliano Allegri rested goalkeeper Gianluigi Buffon, Andrea Barzagli and Paulo Dybala.
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Gaston Ramirez and Fabio Quagliarella fired Sampdoria to a 2-0 Serie A win at Genoa in the Derby della Lanterna.
Inter Milan went top of Serie A after holding on for a 3-2 win against Sampdoria at the San Siro.
Udinese ended Sampdoria's unbeaten Serie A start with a 4-0 victory at Friuli, where Blucerchiati midfielder Edgar Barreto was sent off.
Roma's Serie A match at Sampdoria on Saturday has been postponed due to severe thunderstorms forecast in Genoa.
Roma have announced the signing of Patrik Schick from Sampdoria on a five-year contract.
Patrik Schick is set to become Roma's record signing after arriving in the Italian capital for a medical ahead of a reported €40 million move.
Czech Republic international saw a proposed move to Juventus fall through earlier this summer after he failed a medical.
Sampdoria president Massimo Ferrero says Roma are leading the chase for Patrik Schick, who has also been linked with a move to Inter Milan.
Juventus' bid to sign striker Patrik Schick from Sampdoria has fallen through, his club announced on Tuesday.
Wesley Sneijder has joined Ligue 1 side Nice on a free transfer.
Ramirez, a Uruguay international, made 44 appearances for Boro after joining them from Southampton, initially on a loan basis, last year.