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Nice striker Balotelli was booked for gesture towards Dijon supporters during 3-2 defeat last month.
Representative Council of Black Associations (CRAN) filed complaint against "persons unknown" after Balotelli said he was target of monkey chants.
Monaco solidified second position in Ligue 1 with a 4-0 rout of Dijon on Friday night at the Stade Louis II stadium.
The French Football League has opened a formal investigation into alleged racist abuse aimed at Mario Balotelli with a final report due in mid-March.
The referee who booked Mario Balotelli after the Nice striker alleged he had been the victim of racist abuse has insisted he did not hear the insults.
The referee who booked Mario Balotelli for making a gesture toward Dijon fans after being subjected monkey chants is "doubly guilty," CRAN has said.
LFP tells AFP "the matter will be studied by the Disciplinary Commission" on Thursday.
Dijon striker Wesley Said and Kick It Out each condemned the decision to book Nice's Mario Balotelli after he was victimised by racist abuse.
The world's most expensive player Neymar plundered four goals as Paris St Germain reasserted their dominance of Ligue 1 with a 8-0 thrashing of Dijon.
Wesley Said's late goal earned Dijon a fifth win in seven Ligue 1 games as Malcom's stunner was in vain for Bordeaux.
Thomas Meunier scored twice to send Paris Saint-Germain six points clear at the top of Ligue 1 with a dramatic 2-1 win at struggling Dijon.
Sunderland have announced that defender Papy Djilobodji has joined Dijon on a season-long loan.
Radamel Falcao netted a hat trick while Kylian Mbappe watched from the bench as champions Monaco made it two wins out of two Ligue 1 games so far.
Monaco restored their three-point lead over Paris Saint-Germain at the summit of Ligue 1 after coming from behind to defeat Dijon 2-1.
Monaco extended their lead at the top of Ligue 1 as Radamel Falcao's second-half goal secured a 1-0 win at Angers.
Marseille had to settle for a point in a 1-1 draw at home to Ligue 1 strugglers Dijon.