Swiss Federal Tribunal said a judge had dismissed Paolo Guerrero's request to provisionally lift the suspension.
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Nico Schulz scored on his debut to help Germany avoid a home draw against Peru in an international friendly on Sunday.
The U.S. men's national team will play Peru in an exhibition on Oct. 16 at Pratt & Whitney Stadium at Rentschler Field East Hartford, Connecticut.
Peru captain Paolo Guerrero, who was cleared to play at the World Cup by Switzerland's supreme court, has again been blocked from playing.
Peru striker Paolo Guerrero has signed a three-year deal with Internacional, the Brazilian club said on Sunday.
Argentine Ricardo Gareca has signed a new three-year contract to lead Peru's national team through qualifying for the 2022 World Cup in Qatar.
The so-called Clasico del Pacifico had been expected to be played in Peru but will be held at the Hard Rock Stadium.
The chairman of Peru's football federation is under pressure to resign after being linked to allegations of illicit favors to a judge.
A fan took out a page of personal ads in a Buenos Aires paper in an unusual appeal for Peru's Argentinian coach, Ricardo Gareca, to stay on the job.
Sampaoli, who took over last summer, left his job two weeks after the World Cup round-of-16 defeat to France.
The Argentina Football Association (AFA) parted company with Sampaoli on Sunday, 14 months after he took over the national team.
The Seattle Sounders completed the signing of Raul Ruidiaz on Friday, after the forward finished playing at the World Cup with Peru.
The World Cup has been superb on the pitch but equally brilliant off it thanks to the rich, vibrant and merry travelling fans who came to party.
Peru were already out of the World Cup before they beat Australia 2-0 but the victory was not enough to shake a bitter taste for Andre Carrillo.
Australia's World Cup adventure ended with a limp defeat to a Peru side who scored the goals their vibrant fans deserved.
Australia's slim hopes of advancing from Group C were extinguished on Tuesday with a 2-0 defeat to Peru, who exited the World Cup on a high note.