The sisters received a doubles wild card from the tournament on Saturday, allowing them to play in that event after skipping it in 2011 and 2012.
It's only the fourth time they'll be a pair at Roland Garros. They won the French Open doubles title in 1999 -- beating Martina Hingis and Anna Kournikova in the final -- and again in 2010. They lost in the third round in 2009.
The Williams sisters own a total of 13 Grand Slam trophies from women's doubles to go along with the 15 won by Serena and seven by Venus in singles.