Notre Dame has suspended running back Cierre Wood and defensive end Justin Utupo for the first two games of the season, the school announced Sunday.
Neither player will travel to Saturday's opener against Navy in Dublin, Ireland.
"Our players are aware of the standards I set for our program and that failure to meet my expectations will result in consequences," coach Brian Kelly said in a statement. "Justin and Cierre violated our team rules so I've suspended them for the first two games of the season."
Wood, a senior, rushed for 1,102 yards and nine touchdowns last season. Theo Riddick, who has played both running back and wide receiver, likely will replace Wood as the Irish's No. 1 running back during the first two games.
Utupo, a junior, has seen mostly special teams action for the Irish during his career.
The players also will miss the Sept. 8 home game against Purdue.
Earlier, Kelly suspended quarterback Tommy Rees and linebacker Carlo Calabrese for the season opener for their roles in a skirmish with police following an off-campus party in May.
The Irish also have lost starting cornerback Lo Wood for the season with a ruptured Achilles tendon. He underwent surgery on Wednesday.
Information from The Associated Press was used in this report.