Austin pulled up lame after a 37-yard catch in the second quarter of Sunday's 23-13 win and did not return to the game. He was replaced by Laurent Robinson, who had five catches for 32 yards and a touchdown. Austin finished with two catches for 53 yards.
Austin will have an MRI on Monday.
He said the injury to his right hamstring is higher than the one to his left, which kept him out of two regular season games.
"It's really early," Austin said after the game. "I need to get an MRI and wake up in the morning and see how I feel. I can't say I'll be back and practicing on Thursday or playing. It's very surprising. You don't expect something like that but the other guys stepped up as they should and I'm going to try to get back. It's paramount I try to get back as quick as I can."
Austin suffered the left hamstring injury in training camp and sat out a few workouts and then re-injured it at San Francisco in Week 2. He missed the next two games before returning Oct. 16 at New England.
"It's going to be hard without him because he's an explosive (threat) that's outstanding at that position, so you're going to miss him for sure," quarterback Tony Romo said. "But we've got some depth there. We've got guys that have picked it up when he's been out."
Todd Archer covers the Cowboys for ESPNDallas.com.