The 37-year-old Bryant reported issues with his shoulder after the Lakers' win in Boston on Wednesday. He then sat out Friday's win over the Philadelphia 76ers.
The Lakers announced Bryant's status for Sunday's game about an hour before tipoff.
Bryant tore the rotator cuff in his shoulder in late January 2015 during a dunk in New Orleans. After he played just 35 games, the injury sidelined him for the rest of the 2014-15 season.
Bryant is averaging 17.2 points, 4.2 rebounds and 3.4 assists in 30.4 minutes per game this season, his 20th in the NBA. He has said he plans to retire at the end of this season.