He was hurt running the bases in Monday's 8-4 win over the Texas Rangers. He described the injury as a mild strain that will take about five days to a week to heal.
Duffy missed all of last season because of Achilles tendon surgery and a heel injury.
The Rays purchased the contract of Brandon Snyder from Triple-A Durham, where the infielder hit .154 with a homer and three RBIs in eight games.
Tampa Bay also transferred center fielder Kevin Kiermaier from the 10- to 60-day disabled list. Kiermaier is to have surgery Friday for a torn ligament in his right thumb and will be out two to three months.