Altuve beat out an infield single in the first inning. With a 3-0 count on the next batter, manager AJ Hinch and a trainer went out to talk to him. After a short conversation, Altuve walked off the field.
Hinch said after the game that Altuve looked uncomfortable at first and he was working to stretch his hamstring.
"He just said that he felt something in his left hamstring," Hinch said. "He's been evaluated. It doesn't seem serious. We don't know the extent of the injury until we get to tomorrow. He was frustrated, but he made the right decision to alert us of some soreness."
Aledmys Diaz pinch-ran for Altuve and replaced him at second base.
Altuve entered the game batting .238 with nine home runs and 21 RBIs. He has batted .149 (10-for-67) since April 19.
The Associated Press contributed to this report.