Mets manager Mickey Callaway said before the game that Cano was being evaluated and the team hoped he would be available off the bench Thursday afternoon to face the Giants. Cano, however, didn't appear in the game, won by the Mets 7-3.
The 36-year-old Cano came off the injured list Wednesday. He'd been out since May 22 because of a strained left quadriceps.
Cano exited after four innings, limping off the field with tightness in his quad.
Cano is hitting .238 with three home runs and 14 RBIs in his first season with the Mets.
The Associated Press contributed to this report.