Thunder coach Scott Brooks told reporters that Durant would miss the game after the team's shootaround Wednesday morning. The reigning NBA MVP still is considered day to day with a sprained big toe on his left foot.
Russell Westbrook scored 40 points on 13-of-30 shooting for the Thunder in Wednesday night's loss, but they didn't have nearly enough balance without Durant and fell to 23-23.
Despite the absence of their leading scorer, the Thunder defeated the Timberwolves 92-84 thanks in part to a double-double by Serge Ibaka.
Durant, who also missed the first month of the season with a foot injury, is averaging 25.6 points and 6.7 rebounds per game.
The Associated Press contributed to this report.