Booker previously entered the protocols this season in September and missed media day and the start of training camp.
Booker is a three-time All-Star and the team's leading scorer, averaging 25.6 points, 5.2 rebounds and 4.6 assists per game. The 25-year-old's absence is another blow to the Suns, who are also missing fellow All-Star guard Chris Paul.
Paul broke his thumb Feb. 16 and could miss the rest of the regular season, though the team is hopeful he will return before the playoffs.
With Wednesday's win, the Suns became the first NBA team to 50 wins this season and need to play just .500 ball the rest of the regular season to reach 60 wins.
The Associated Press contributed to this report.