After sitting out Wednesday's practice, Wade spoke to reporters and closed the session by telling them, "I won't be seeing you for a little while."
The Heat have listed him as day-to-day, but teammate Chris Bosh hinted that Wade's absence may last weeks.
"It really sucks sometimes, but what can you do?" Bosh said of Wade's latest injury. "We haven't had our whole team. I just want to play with our whole team, just once. But it's not going to be for [Friday], maybe not for the next couple weeks, but there's nothing we can do about that."
Wade injured his right hamstring in Tuesday's loss to the Milwaukee Bucks. Wade said he tweaked it on a play where he was defending a dunk by Bucks guard Brandon Knight. He said he tried to play through it for a few plays.
"And then it just went," Wade said of his right hamstring.
Wade missed two games earlier this season with a right hamstring injury and missed another seven games with a strained left hamstring.
"This is more in the belly of the hamstring so this is a little tougher one," Wade said, comparing this current injury with his previous hamstring issues.
The Heat are 20-25 this season and currently reside in the seventh slot in the Eastern Conference with the Charlotte Hornets, Brooklyn Nets and Boston Celtics trailing within three games in the loss column. Heat forward Luol Deng is also listed as out for Friday's game with a strained calf.
"It's not an ideal situation for none of us," Wade said. "I know our fans are very frustrated. I just want them to know no one is more frustrated than we are. Because I want to be out there."
The injury comes on the same day Wade was named an All-Star reserve, along with Bosh. While he will be at All-Star Weekend in New York City, Wade said he doesn't know if he would play.
The game takes place a little more than two weeks away on Feb. 15. With the extended week-long All-Star break, the Heat have nine days off between games at Cleveland on Feb. 11 and at New York on Feb. 20.
Wade is averaging 21.4 points and 5.4 assists per game this season on 48.7 percent shooting, but he has missed 10 games with various injuries.