The injury has been evaluated as a high ankle sprain, a league source confirmed. Barbre is expected to see a specialist soon who will further evaluate the injury.
Barbre had been starting for suspended right tackle Lane Johnson.
The nature of Barbre's injury was reported earlier Monday by the Philadelphia Inquirer.
The Eagles have nine offensive linemen on their 53-man roster, but only six are currently healthy. Guard/tackle Matt Tobin (ankle) is expected to return to practice this week.
Starting left guard Evan Mathis (sprained knee) had an MRI on Monday morning, but the results are not currently available. A source confirmed that he has a sprained MCL but the severity of the injury isn't yet clear.
Both Barbre and Mathis were injured in the first half as the Eagles went into halftime trailing 17-0 to the Jacksonville Jaguars. The Eagles posted a 34-point second-half comeback with backup center David Molk playing left guard, a position he said he hadn't practiced at all summer, and Andrew Gardner, who played mostly at left tackle during the preseason, filling in for Barbre at right tackle.
Quarterback Nick Foles was sacked five times in the first half, most of which was played with the starting line in front of him. In the second half, with Molk and Gardner filling in, Foles wasn't sacked at all.
The Eagles dressed seven offensive linemen Sunday. Two were inactive. Coach Chip Kelly said he likely would have used a tight end, probably Brent Celek, if another linemen had been injured.
Information from ESPN.com Eagles reporter Phil Sheridan is included in this report.