The ruling was made Thursday by U.S. District Court Judge Anne C. Conway in regard to the civil suit brought by Erica Kinsman, who accused Winston of rape in 2012 while he attended Florida State.
Winston was investigated by Tallahassee and Florida state police in the case, but he wasn't charged with a crime.
Kinsman filed the lawsuit in state court in Orlando in April. According to court documents, she accused Winston of sexual battery, assault, false imprisonment and intentional infliction of emotional distress arising out of forcible rape.
A resident of Alabama at the time, Winston sought a motion to transfer the case to U.S. District Court for the Northern District of Florida in Tallahassee.
Conway ruled that because both parties lived in Central Florida, there was no reason to move the case to Tallahassee. The judge also ruled it would be less convenient for witnesses to hold the civil trial in Tallahassee.
Late Thursday afternoon, Winston, the No. 1 overall pick in this year's draft, tweeted a picture of himself working out with Tampa Bay wide receivers Mike Evans and Louis Murphy, a seven-year veteran out of Florida.
Winston showed the same kind of work ethic during offseason workouts. He took double repetitions during one practice to make up the time he missed while attending the NFLPA Rookie Premier event in Los Angeles.
Winston was so dedicated putting in extra time in May that coach Lovie Smith told ESPN.com's Pat Yasinskas, "We have to tell him when to leave."