Trufant initially was questionable to return to the game, but the Falcons ruled him out at halftime.
"We don't know the extent of it yet, so we're hopeful that it's not serious," Quinn said after the game. "We'll get an MRI [Friday] and then we'll come back, and obviously [Friday], we'll have more information for you regarding that."
Before exiting, Trufant shadowed Buccaneers wide receiver Mike Evans. Evans appeared to get the better of Trufant, with five catches for 46 yards and a touchdown on six targets when defended by him, according to Pro Football Focus.
With Trufant out, Jalen Collins stepped in at corner and made some physical plays, including a big hit on Bucs quarterback Jameis Winston to prevent a two-point conversion with seven minutes left in the game.
Collins, a second-round draft pick from last season who was inactive just last week, was suspended for the first four games of the season for violating the league's policy on performance-enhancing substances.
Robert Alford took over as the Falcons' lead cornerback.