He said he expects to have an MRI on Monday morning in Nashville and doesn't know the extent of the injury.
"Warming up, I just pulled my calf," Henry told ESPN on his way to the team bus at Qualcomm Stadium. "I'm not sure. I'll go to the trainers, have an MRI."
The Titans expected to have to throw the ball against the Chargers because of the San Diego run defense. And they did not have much success on the ground.
Quarterback Marcus Mariota carried three times, scoring on an impressive 14-yard touchdown run but also fumbling on an option run. Chargers defensive back Dwight Lowery scooped that up and went 36 yards for a score.
He had 16 carries for 60 yards and his first NFL touchdown as well as four catches for 37 yards.
Titans coach Mike Mularkey said after the game that the team's running back workload would continue to be determined by the flow of the game.
The Titans host the Packers next Sunday.