The Packers downgraded Allison from doubtful on Saturday, when the team had a light practice. Allison did not travel to New England.
He was a key contributor in the passing game before missing two weeks with a hamstring injury. He had one catch for 14 yards in returning against the Rams last week, then hurt his groin this week in practice.
Allison has 20 catches for 303 yards and two scores. His absence likely means more playing time for rookie Marquez Valdes-Scantling, who has touchdown catches in two of his last three games.
The Packers added reserve cornerback Tony Brown to the injury report as questionable with a hip injury.
Green Bay also signed punter Drew Kaser. The Packers are not moving on from incumbent punter, rookie JK Scott, but it remains to be see if he'll punt Sunday night. Scott's 25-yard punt set up the Rams' game-winning field goal last week.
They were at 52 on the roster because they had not filled the spot that opened when they traded Ha Ha Clinton-Dix on Tuesday.
Information from ESPN's Rob Demovsky and The Associated Press was used in this report.