The move comes a day after quarterback Matthew Stafford was also moved from the same list to the active roster.
Golladay was one of the first Lions players to be placed on the list -- one of five Detroit players to be placed there last Wednesday. He is the first from that group to be activated.
The fourth-year receiver was a Pro Bowler last year and is entering a contract year. Lions general manager Bob Quinn declined to discuss any negotiations with Golladay when Quinn spoke with the media last week.