Chark was questionable with a chest/back injury, which he suffered in the Jaguars' loss to the Tennessee Titans on Sunday. He was hurt when he hit the ground hard on a leaping 45-yard catch.
Chark being out likely means that fourth-year wide receiver Dede Westbrook will be active Thursday for the first time this season. He was a healthy scratch the first two games.
Chark has seven catches for a team-high 109 receiving yards and a touchdown this season.