Wylie was taken to a Baltimore hospital before the game with a stomach illness that doctors at first suspected could be appendicitis, according to the team.
After the game, coach Andy Reid said that Wylie underwent some tests and "things worked out OK," but noted that Wylie is "not feeling very well."
Wylie, 26, started 21 games for the Chiefs over the past two seasons.
Veteran Mike Remmers, signed as a free agent during the offseason, started in Wylie's place at right guard.
Chiefs starting cornerback L'Jarius Sneed left the game with a right shoulder injury. According to Reid, he will have more tests Tuesday "just to see what exactly happens there."
Reid also said defensive tackle Chris Jones tweaked his groin toward the end of the game.