Mitchell, who had been listed as questionable with a finger and rib injury, underwent surgery on the finger Nov. 16. He suffered a rib injury against the Chicago Bears on Oct. 31 but did not miss time until he hurt the finger.
He was limited in practice this week, but Niners coach Kyle Shanahan on Friday said the rookie was moving in the right direction.
A sixth-round pick out of Louisiana in this year's draft, Mitchell has established himself as the 49ers' top running back. In his seven games, Mitchell leads the 49ers in carries (116) and rushing yards (560), and he is 12th in the NFL in yards per carry (4.8).